Pierce ProFound™ GST Protein Interaction Pull Down Kit, Thermo Scientific
Supplier: 0000013509
The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.
- Complete kits provide all components and detailed protocols for purifying protein:protein interactions
- No special equipment needed – use common laboratory equipment and reagents
- Microcentrifuge spin columns facilitate simple and efficient manipulation of agarose beads, including simple processing of multiple samples
- Instructions include protocols for use with bait and prey proteins expressed from a variety of sample types
- Cobalt chelate resin is more specific for histidine-tagged fusion proteins than nickel resins, resulting in less non-specific binding
- Binds 10 to 25 mg of histidine-tagged fusion protein per mL of resin
Applications include discovering a new protein:protein interaction from a cell lysate; confirming a putative interaction from a cell lysate or with a previously purified protein; extracting protein:protein interaction information from in vitro transcription/translation lysates.
Delivery information: Complete kit includes Cobalt chelate agarose resin, (750 µL); Pull-Down Lysis Buffer, (250 mL); Lysis buffer, (250 mL); TBS pack (makes 500 mL); Spin columns and accessories, (27 columns); and Microcentrifuge collection tubes, (100 tubes).
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