Pierce™ Monomeric Avidin Agarose Kit, Thermo Scientific
Supplier: 0000013509
The Thermo Scientific Pierce™ Monomeric Avidin Kit enables mild affinity purification of biotinylated proteins, peptides and other molecules
- Monomeric Avidin Agarose can be regenerated and reused at least 10 times
- Retains biotin-binding specificity and low nonspecific binding of native avidin
- Bind samples in a variety of physiological buffers
- Binding capacity >1.2 mg biotinylated BSA/mL resin
During monomeric avidin immobilization, polymeric forms of avidin with strong binding characteristics also are immobilized. These high affinity biotin-binding sites must first be blocked with a biotin-containing buffer. Biotin molecules are eluted from monomers with a glycine solution revealing only the reversible binding sites. The biotinylated molecule of interest may then be applied to the support for purification and then eluted by ligand competition using a biotin-containing solution.
Avidin monomers have a much lower biotin-binding affinity than native tetrameric avidin enabling dissociation of biotinylated molecules using mild elution conditions
Delivery information: Kit includes Pierce™ Monomeric Avidin column, 2 mL (1 column), PBS pack, makes 500 mL (1 pack), Biotin Blocking and Elution Buffer (200 mL), Regeneration Buffer (250 mlL, and column accessories.
Caution: For Research Use Only. Not for use in diagnostic procedures.
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