Pierce™ Magnetic RNA-Protein Pull-Down Kit, Thermo Scientific
Supplier: 0000013509
The Thermo Scientific Pierce Magnetic RNA-Protein Pull-Down Kit provides researchers with a streamlined, robust method to enrich protein-RNA interactions using end-labeled RNA as the bait.
- Capture ribonucleoprotein complexes directly with end-labeled RNA
- Does not use or require antibodies for pull-down
- No spin cups or centrifugation necessary for the enrichment of RBP
- Use in vitro transcribed RNA or synthetic RNA for labeling of various lengths and complexity
- Specific—low bead background; unrelated RNA or mutated RNA does not significantly enrich specified RBPs
Magnetic RNA-Protein Pull-Down Kit provides reagents to efficiently enrich RNA Binding Proteins (RBPs) using RNA end-labeled with desthiobiotin and streptavidin magnetic beads. This direct enrichment of the protein-RNA interaction provides an alternative to antibody capture of protein-RNA complexes or moieties incorporated into the nucleic acid. The kit is amenable to several downstream applications, including Western blotting and mass spectrometry. It also finds applications in mutational analysis, structure function analysis and identification of RNA-protein interactions and MS analysis of unknown RNA-protein binding pairs or complexes.
Delivery information: The kit includes Pierce™ Nucleic acid-compatible streptavidin magnetic beads (1 mL), RNA capture buffer (1X) (10 mL), Tris (20 mM, pH 7,5) (5 mL), Protein-RNA binding buffer (10X) (1 mL), Wash buffer (1X) (10 mL), Biotin elution buffer (1,5 mL), HuR monoclonal antibody (mouse) (50 µL), Positive RNA control (AR RNA) (250 pmol), Negative RNA control (polyA25 RNA) (250 pmol) and Pierce™ RNA 3' end desthiobiotinylation kit.
Caution: For Research Use Only. Not for use in diagnostic procedures.
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