HisProbe™-HRP Conjugate, Thermo Scientific

Supplier: 0000013509

15165
PI15165EA 363.14 USD
PI15165
HisProbe™-HRP Conjugate, Thermo Scientific
Electrophoresis Reagents Western Blotting Reagents

Thermo Scientific HisProbe-HRP is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.


  • One-step probe incubation eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
  • Recognize polyhistidine tags independent of adjacent tags
  • Smaller than antibodies and detects his tagged fusion proteins that are often undetectable


Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.


Caution: For Research Use Only. Not for use in diagnostic procedures.

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