The Thermo Scientific QuantaRed Enhanced Chemifluorescent HRP Substrate (ADHP) is the most sensitive fluorescent ELISA substrate, providing sensitivity comparable to enhanced chemiluminescence.
- Low picogram to femtogram detection
- Minimal autofluorescence interference from biological samples
- Use Stop Solution to effectively stop the reaction resulting in stable signal for at least 4 hours or more.
- Produces a strong colorimetric signal and can be read fluorescently or colorimetrically at 570nm
ADHP is a non-fluorescent compound that reacts with horseradish peroxidase (HRP) to produce resorufin, a soluble, highly fluorescent reaction product with excitation/emission maxima of ~570/585nm. The long wavelength emission of the resorufin minimizes interference from the low wavelength (blue and green) autofluorescence, making it an ideal substrate for quantitating target molecules in biological samples. In addition, the reaction product of QuantaRed Substrate is colorimetric as well as being fluorescent and can be read spectrophotmetrically at ~570nm.
Ordering information: Sufficient For: 960 wells (10 x 96-well plates) at 100 µL working solution per well
QuantaRed ADHP Concentrate, 1 mL
QuantaRed Enhancer Solution, 50 mL
QuantaRed Stable Peroxide Solution, 50 mL
QuantaRed Stop Solution, 10 mL
Store at 4 °C.
Delivery information: Kit includes QuantaRed™ ADHP Concentrate (1 ml), QuantaRed™ Enhancer Solution (50 ml), QuantaRed™ Stable Peroxide Solution (50 ml), QuantaRed™ Stop Solution (10 ml).
Caution: For Research Use Only. Not for use in diagnostic procedures.
