E. coli S30 Extract System for Linear Templates, 30 Reactions, Promega
Supplier: Promega Corporation
The E. coli S30 Extract System for Linear Templates allows transcription/translation of linear DNA templates.
- Successful Transcription/Translation of Linear DNA Templates
The E. coli S30 Extract System for Linear Templates allows successful transcription/translation of linear DNA templates. The investigator need only provide linear DNA containing a prokaryotic E. coli-like promoter (such as lacUV5, tac, lambdaPL (con) and lambda-P(R)). A ribosome binding site is required to direct the synthesis of proteins in vitro. In vitro-generated RNA from DNA templates lacking an E. coli promoter may also be used in this system, but protein yields will be decreased to 1-10% of that produced from linear DNA templates.
Applications for this system include identification of cloned or synthesized genes from linear or closed circular DNA and rapid structure mapping of genes without additional cloning steps.
coli-like promoter (such as lacUV5, tac, λPL (con) and λ-PR). A ribosome binding site is required to direct the synthesis of proteins in vitro. In vitro-generated RNA from DNA templates lacking an E. coli promoter may also be used in this system, but protein yields will be decreased to one to 10% of that produced from linear DNA templates.
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