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JM109 Competent Cells, >10(7)cfu/ug, Promega

Supplier: Promega Corporation

JM109 Competent Cells, >10(7)cfu/ug, Promega

JM109 Competent Cells, >10(7)cfu/ug, Promega

L1001

PAL1001EA 161.85 USD

PAL1001

JM109 Competent Cells, >10(7)cfu/ug, Promega

Competent Cells

JM109 is a K strain that is recA- and endA- to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening.

Ready-to-Use Competent E. coli Cells for Recombinant Cloning and Subcloning
Cells are ready to use—no preparation time necessary
Transformation efficiencies guaranteed
No worries about undesirable recombination events (recA- mutation), and no nuclease carryover in miniprep DNA (endA- mutation)

JM109 is a K strain that is recA- and endA- to minimize recombination and improve the quality of plasmid DNA. In addition, the cells carry the F´ episome, which allows blue/white screening. JM109 Competent Cells are available for convenient transformation in two efficiencies: High Efficiency at greater than 10(8)cfu/ug and Subcloning Efficiency at greater than 10(7)cfu/ug. JM109 Competent Cells are prepared according to a modified procedure of Hanahan. JM109 Genotype: endA1, recA1, gyrA96, thi, hsdR17 (r(k)-, m(k)+), relA1, supE44, Delta(lac-proAB), [F' traD36, proAB, laqI(q)ZDeltaM15].

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