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pCI-neo Mammalian Expression Vector, 20 µg, Promega

Supplier: Promega Corporation

pCI-neo Mammalian Expression Vector, 20 µg, Promega

pCI-neo Mammalian Expression Vector, 20 µg, Promega

E1841

PAE1841EA 545.85 USD

PAE1841

pCI-neo Mammalian Expression Vector, 20 µg, Promega

Vectors, Plasmids and Libraries

Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. Transfected cells can be selected with G-418.

Constitutively Express Cloned Insert for Transient or Stable Transfection
Select for stably transfected cells with neomycin phosphotransferase gene
Increase the steady-state level of RNA with the late SV40 polyadenylation signal approximately fivefold more than the early SV40 polyadenylation signal
Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate ssDNA in E. coli using the f1 origin of replication

The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418.

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