pBIND-ER(alpha) Vector, 20 µg, Promega

Supplier: Promega Corporation

E1390
PAE1390EA 545.85 USD
PAE1390
pBIND-ER(alpha) Vector, 20 µg, Promega
Vectors, Plasmids and Libraries

NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.


  • Vectors To Bind Nuclear Receptors Using Recognition Sequences or One-Hybrid Assay
  • Create a binding domain fusion with the pFN26 (BIND) hRluc-neo Flexi(R) Vector and use with the pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector
  • Mouse mammary tumor virus long terminal repeat in pGL4.36[luc2P/MMTV/Hygro] Vector assesses androgen or glucocorticoid responses
  • The estrogen receptor ligand binding domain (pBIND-Er∝) and the glucocorticoid receptor ligand binding domain (pBIND-GR) are already cloned


Nuclear receptor analysis can be performed with traditional means using a minimal promoter vector with nuclear receptor response elements upstream. Alternatively, you can use viral elements like the mouse mammary tumor virus long terminal repeat promoter to judge androgen or glucocorticoid responses (e.g., pGL4.36). In many cases, use of these methods requires a cell line with the appropriate endogenous nuclear receptors, meaning you may need different cell lines for each nuclear receptor study. A method using the principles of the yeast two-hybrid system was adapted for nuclear receptor work. The nuclear receptor ligand binding domain is fused to the GAL4 DNA binding domain and transfected with a firefly luciferase vector containing repeats of the GAL4 upstream activation sequence upstream of a minimal promoter. The ligand binding domain is responsible for ligand binding, homo- or heterodimerization and interactions with co-activator or co-repressors. The one-hybrid method allows you work with any cell line and nuclear receptor you desire.

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