GoTaq Probe 2-Step RT-qPCR System, 2ml, Promega®
Supplier: Promega Corporation
GoTaq Probe RT-qPCR Systems enable detection and relative quantification of RNA expression levels.
- Robust System to Simplify RNA Expression Analysis
- Integrated components for reverse transcription and downstream qPCR, including RNasin(R) Ribonuclease Inhibitor
- Compatible with both fast and standard cycling methods using probe-based assays
- Sensitive detection for quantitation over a broad dynamic range
The GoTaq Probe 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript Reverse Transcription System and GoTaq Probe qPCR Master Mix. The GoScript Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions. The GoTaq Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript Reverse Transcriptase and GoTaq Probe qPCR Master Mix in a single-step real-time amplification reaction.
Caution: Store all components between –30°C and –10°C. Protect components from light at all times. For best results, mix thawed solutions gently to minimize aeration and foaming, and keep on ice. For short–term storage and frequent use, the GoTaq qPCR Master Mix, 2X, may be kept at 2 to 8°C for up to three months if protected from light.
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