Human IgG1 ELISA Kit
Supplier: CLOUD-CLONE CORP MS
This assay has high sensitivity and excellent specificity for detecting Human IgG1 (Immunoglobulin G1). The assay range is from 6.25 to 400 ng/ml (Sandwich kit) with a sensitivity of 2.83 ng/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.
- High sensitivity and specificity
- Perfect reproducibility and consistency across batches
- Quality control with three-level inspections
- Wide range of targets/species available
- Intra-assay: CV<10%; Inter-assay: CV<12%
IgG is the predominant immunoglobulin in the serum and has a molecular weight of about 150 kDa. Four distinct subgroups of human IgG (IgG1, IgG2, IgG3, and IgG4) were first demonstrated in the 1960's by using polyclonal antisera prepared in animals immunized with human myeloma proteins. Quantitatively, the relative serum concentrations of the IgG subclasses are as follows: IgG1>IgG2>IgG3 = IgG4. The four IgG subclasses show their most conspicuous differences in the amino acid composition and structure of the 'hinge region', which is the part of the molecule containing disulfide bonds between the y-heavy chains. The hinge region of IgG1 encompasses amino acids 216-231 and since it is freely flexible, the Fab fragments can rotate about their axes of symmetry and move within a sphere centered at the first of two inter-heavy chain disulfide bridges.
Caution: For research use only. Not for use in clinical diagnostic procedures. Please proper stored each component based on the instruction.
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