General Species H6 ELISA Kit

Supplier: CLOUD-CLONE CORP MS

CEX656GE
MSPP-CEX656GEEA 812 USD
MSPP-CEX656GE
General Species H6 ELISA Kit
Assays ELISAs

This assay has high sensitivity and excellent specificity for detecting General species H6 (Hexa Histidine). The assay range is from 24.69 to 2000 pg/ml (Competitive kit) with a sensitivity of 8.81 pg/ml. There is no detectable cross-reactivity with other relevant proteins. Activity loss rate and accelerated stability test ect have been conducted to guarantee the best performance of the products after long storage and delivery.


  • High sensitivity and specificity
  • Perfect reproducibility and consistency across batches
  • Quality control with three-level inspections
  • Wide range of targets/species available
  • Intra-assay: CV<10%; Inter-assay: CV<12%


The hexahistidine (His(6))/nickel(II)-nitrilotriacetic acid (Ni(2+)-NTA) system is widely used for affinity purification of recombinant proteins. The NTA group has many other applications, including the attachment of chromophores, fluorophores, or nanogold to His(6) proteins. Its high specificity makes (Ni(2+)-NTA)(2)-Cy3 ideal for detecting His(6) proteins in complex mixtures of other proteins, allowing (Ni(2+)-NTA)(2)-Cy3 to be used as a probe in crude cell extracts and as a His(6)-specific gel stain. (Ni(2+)-NTA)(2)-Cy3 binding is reversible in 10 mM ethylenediaminetetraacetic acid (EDTA) or 500 mM imidazole, but in their absence it exchanges slowly. Labeling with (Ni(2+)-NTA)(2)-Cy3 allows characterization of hydrodynamic properties by fluorescence anisotropy or analytical ultracentrifugation under conditions that prevent direct detection of protein.


Caution: For research use only. Not for use in clinical diagnostic procedures. Please proper stored each component based on the instruction.

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