Blasticidin S hydrochloride solution 10 mg/ml in HEPES ≥95% (by HPLC)
Supplier: Invivogen
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Danger
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Blasticidin hydrochloride is supplied as a sterile filtered solution at 10 mg/ml in HEPES buffer.
Blasticidin is a selection antibiotic that acts on both eukaryotic and prokaryotic cells. It is a peptidyl nucleoside antibiotic isolated from the culture broth of S. griseochromogenes. It specifically inhibits protein synthesis in both prokaryotes and eukaryotes by inhibiting peptide bond formation in the ribosomal machinery.
Three blasticidin resistance genes have been cloned and sequenced that are an acetyl transferase gene, bls from a blasticidin producer strain and two deaminase genes, bsr gene from B. cereus and BSD gene from A. terreus. Both bsr and BSD genes are used as dominant selectable markers for gene transfer experiments in mammalian and plant cells. Blasticidin was developed as a selection agent for mammalian cells, it can also be used in E. coli.
Formula:
C₁₇H₂₇ClN₈O₅ Storage Temperature: Refrigerator |
MDL Number:
MFCD02091640 CAS Number: 3513-03-9 UN: 2810 ADR: 6.1,III |
Specification Test Results
Purity (HPLC) | ≥95% |
Endotoxin level | <1 EU/mg |
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