Dithiothreitol (DTT, Cleland's reagent) ≥99.5%, white powder for electrophoresis
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
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Warning
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Synonyms:
cis-2,3-Dihydroxy-1,4-butanedithiol, (R*,R*)-1,4-Dimercapto-2,3-butandiol, DTT, DL-1,4-Dithiothreitol
DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.
- Electrophoresis Grade
- Half Life: 1.4( pH 8.5 at 20 °C)
- pH: 4.0 to 6.0 (0.1 M in H2O, 25 °C)
- Redox potential = -0.33 volts at pH 7
- Soluble in water (50 mg/mL - clear and colorless solution); soluble in ethanol, acetone, ethylate, chloroform and ether.
- +4 °C
Sulfhydryl reducing agent
Blotting
Protein Purification
DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8. Half life at pH 6.5 and 20 °C was found to be 40 hours.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).It is used in preparation of carboxymethylated rPA
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Formula:
C₄H₁₀O₂S₂ MW: 154.25 g/mol Boiling Pt: ? 125 °C (1013 hPa) Melting Pt: 40…50 °C Flash Pt: ≥ 110 °C () Storage Temperature: Refrigerator |
MDL Number:
MFCD00004877 CAS Number: 3483-12-3 EINECS: 222-468-7 UN: 3335 ADR: 9,III |
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Specification Test Results
| Identity Test | Passes |
| Purity | ≥99.5% |
| FTIR | Conforms to Standard |
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