Tris-Glycine Buffer. Powder, MP Biomedicals
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
04816200
IC816200EA
117.05
USD
IC816200
Tris-Glycine Buffer. Powder, MP Biomedicals
Buffers
Electrophoresis Buffers
Transfer Buffers
Tris-Glycine Buffer is used for Western blotting and gel electrophoresis
Empty contents of 1 packet into a 4-liter flask, add 800 mL of methanol and QS to volume with deionized water. Four liters of pH 8.3 buffer is now ready to use.
Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.
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