Leishman's eosin-methylene blue, greenish-black powder

Supplier: MP BIOMEDICALS (FKA ICN BIOMED

Synonyms: Eosin and methylene blue Leishman, Eosin-Methylene blue dye Leishman, Leishman's stain, Leishman stain, Leishman's stain (eosin methylene blue compound)

0215518325
IC15518325EA 76.82 USD
IC15518325
Leishman's eosin-methylene blue, greenish-black powder
Leishman's Eosin-Methylene blue

Leishman's stain, also Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin.


The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. If a working solution is made by dilution with an aqueous buffer the resulting mixture is very unstable and cannot be used for long. Leishman stain is a Romanowsky-type dye, similar to Wright stain.


Leishman's Stain is used generally in hematology and tropical medicine to differentiate and identify leucocytes, malarial parasites and trypanosomes. .


Soluble in water (4 mg/ml), ethanol (4 mg/ml), in 0,1% methanol (clear, blue soln with fluorescence) or methyl cellosolve (20 mg/ml).


Leishman's stain, also Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin. The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. If a working solution is made by dilution with an aqueous buffer the resulting mixture is very unstable and cannot be used for long. Leishman stain is a Romanowsky-type dye, similar to Wright stain.

Storage Temperature: Ambient
MDL Number: MFCD00131498
CAS Number: 12627-53-1

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Specification Test Results

Identity Test Passes
l max (methanol) 645 ± 5 nm

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