Leishman's eosin-methylene blue, greenish-black powder
Supplier: MP BIOMEDICALS (FKA ICN BIOMED
Synonyms:
Eosin and methylene blue Leishman, Eosin-Methylene blue dye Leishman, Leishman's stain, Leishman stain, Leishman's stain (eosin methylene blue compound)
Leishman's stain, also Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin.
The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. If a working solution is made by dilution with an aqueous buffer the resulting mixture is very unstable and cannot be used for long. Leishman stain is a Romanowsky-type dye, similar to Wright stain.
Leishman's Stain is used generally in hematology and tropical medicine to differentiate and identify leucocytes, malarial parasites and trypanosomes. .
Soluble in water (4 mg/ml), ethanol (4 mg/ml), in 0,1% methanol (clear, blue soln with fluorescence) or methyl cellosolve (20 mg/ml).
Leishman's stain, also Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is based on a methanolic mixture of "polychromed" methylene blue (i.e. demethylated into various azures) and eosin. The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. If a working solution is made by dilution with an aqueous buffer the resulting mixture is very unstable and cannot be used for long. Leishman stain is a Romanowsky-type dye, similar to Wright stain.
Storage Temperature:
Ambient |
MDL Number:
MFCD00131498 CAS Number: 12627-53-1 |
Specification Test Results
Identity Test | Passes |
l max (methanol) | 645 ± 5 nm |
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