Arthrobacter ureafaciens Recombinant Sialidase A (from E. coli)
Supplier: AGILENT TECHNOLOGIES, INC (CSD)
The enzyme releases Alpha (2,3)-, Alpha (2,6)-, Alpha (2,8)-, and Alpha (2,9)-linked N-acetylneuraminic acid from oligosaccharides and glycoproteins. It is also capable of releasing N-glycolylneuraminic acid.
- pH Optimum: 6
- Unit definition: One unit is defined as the amount of enzyme required to catalyze the release of 1 µmole of p-nitrophenol from p-nitrophenyl-α-D-N-acetylneuraminic acid per minute at 37° C, pH 5.5.
Break the glycosidic bonds at the terminal residue, and enable glycoanalysis studies
These enzymes are useful in the study of isolated glycans, glycolipids, glycoproteins, or sequencing studies, especially when used in combination.
Agilent Exoglycosidases (formerly ProZyme) are glycoside hydrolase enzymes that cleave the glycosidic bonds at the terminal residues. These enzymes are useful in the study of isolated glycans, glycolipids, glycoproteins. Linkage-specific exoglycosidases may also be used for sequencing of oligosaccharides. When used in combination, exoglycosidases enable flexible analytical tools for glycan analysis.
Source: Recombinant gene from Arthrobacter ureafaciens, expressed in E. coli. Sialidases are also known as neuraminidases.
Specificity: The enzyme releases a(2,3)-, a(2,6)-, a(2,8)-, and a(2,9)-linked N-acetylneuraminic acid from complex carbohydrates. The initial rate of hydrolysis of a(2,6) linkages is reported to be approximately twice that of a(2,3)-linked sialic acid however, in practice, this kinetic selectivity is of little consequence during extended incubations. Effective digestion of glycolipid substrates is facilitated by addition of a detergent, such as sodium taurodeoxycholate to the incubation. Sialidase A is capable of releasing N-glycolylneuraminic acid (Neu5Gc, NGNA) in addition to N-acetylneuraminic acid (Neu5Ac, NANA) [1], although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. The enzyme can also be used to remove sialic acid from gangliosides [3], glycosphingolipids (ceramide and oligosaccharide) with sialic acid. May be used for exoglycosidase sequencing with Sialidase S (GK80021), which is specific for a(2,3)-linked sialic acids. Sialidases are also known as neuraminidase.
Delivery information: Incudes 5x Reaction Buffer B [250 mM sodium phosphate pH 6.0] which when diluted gives 50 mM sodium phosphate pH 6.0.
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