Following electrophoresis, proteins are frequently transferred from the polyacrylamide gel onto PVDF or nitrocellulose membranes for use in immunoblotting or sequencing.
- Convenience of liquid concentrates
- Consistent performance and quality
Although there are different methods by which to achieve transfer, the one most routinely used is electroblotting. Electroblotting utilizes conductive buffers that do not interfere with proteins binding to the membrane and can be performed in a vertical buffer tank or in a semi-dry blotting apparatus. Towbin's Tris-Glycine-Methanol buffer is widely used for both tank and semi-dry transfer, although it can be replaced with CAPS buffer in both cases. CAPS buffer is more suitable for transfer of strongly basic proteins and also for use with blots to be subjected to sequencing.
