Protein Detector BCIP/NBT Western Blotting Kit, KPL

Supplier: KPL

5410-0013
95059-338EA 848.85 USD
95059-338
Protein Detector BCIP/NBT Western Blotting Kit, KPL
Stains Electrophoresis Stains

The Protein Detector BCIP/NBT Western Blot Kit is designed for the colorimetric detection of proteins immobilized on membranes through either electrophoresis or dot blotting


The Protein Detector BCIP/NBT Western Blotting Kit is ideal for routine colorimetric detection of overexpressed protein. It provides sensitivity in the 500 picogram range, and contains ready-to-use reagents for quick and convenient assay preparation. BCIP/NBT Substrate is stable for one year. Both anti-rabbit and anti-mouse antibodies are supplied for versatility.


The combination of highly specific alkaline phosphatase-labeled mouse and rabbit secondary antibodies with a sensitive colorimetric substrate allows rapid and accurate identification of samples. All solutions required for blocking and washing the membrane and for diluting antibodies are provided. For added flexibility, affinity purified anti-mouse and anti-rabbit secondary antibodies conjugated with alkaline phosphatase are provided in stabilized, liquid form for quick dilution. The BCIP/NBT substrate is provided as a convenient, ready-to-use solution, which forms an insoluble purple precipitate when reacted with the AP-labeled antibody.

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