HyClone™ AdvanceSTEM, HEPES Buffer, ES Qualified, HyClone products (Cytiva)

Supplier: Cytiva

SH30851.01
95053-212EA 76.56 USD
95053-212
HyClone™ AdvanceSTEM, HEPES Buffer, ES Qualified, HyClone products (Cytiva)
Buffers Cell Culture Buffers

HyClone AdvanceSTEM ES Qualified HEPES is better at maintaining physiological pH despite changes in carbon dioxide as compared to bicarbonate buffers. Providing optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. HyClone AdvanceSTEM line of stem cell culture products can help you meet those challenges.


  • ES cell-qualified with HyClone's ES Screened FBS — no prescreening required.
  • Mycoplasma-free and sterility tested
  • pH range: 7.2 to 7.5
  • 0.1 µm sterile filtered


Providing optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. HyClone AdvanceSTEM line of stem cell culture products can help you meet those challenges.


Classic culture conditions for 129-derived murine embryonic stem cells (mESCs) include culturing in medium containing DMEM, FBS, LIF and co-culturing with primary mouse embryonic fibroblasts (MEFs). Pluripotent mESCs have a very distinct morphology when cultured under these conditions; growing as tightly clustered colonies with smooth phase bright borders. When co-cultured in ES cell media with a mouse embryonic fibroblast (MEF) feeder layer, these stem cell cultures are suitable for electroporation, expansion, or further experimentation.


Certifications: Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

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