Thrombin
Supplier: Cytiva
Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).
- Enables the site-specific cleavage of fusion proteins containing an accessible thrombin recognition sequence
- Purified from bovine plasma; functionally free of other clotting factors, plasminogen, and plasmin
- Can be used either following affinity purification or while fusion proteins are bound to Glutathione Sepharose™ 4B, Glutathione Sepharose™ High Performance, or Glutathione Sepharose™ 4 Fast Flow
Application: Used for site-specific separation of the GST tag from proteins expressed using pGEX-T vectors.
Cleavage Buffer: PBS 140 mM NaCl, 2,7 mM KCl, 10 mM Na2HPO4, 1,8 mM KH2PO4, pH 7.3
Elution Buffer:50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0
Inhibitors: Pefabloc TH benzamidine, AEBSF, APMSF, antithrombin III, Antipain, α1-antitrypsin, aprotinin, chymostatin, hirudin, leupeptin, PMSF
Molecular Weight: 37000
Unit Definition: One unit of enzyme cleaves 90% of 100 μg of a test GST fusion protein when incubated in 1X PBS at 22 °C for 16 hours
Ordering information: Supplied as lyophilized powder. Includes 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0.
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