Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media, Cytiva
Supplier: Cytiva
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
- Phenyl immobilized on 4% cross-linked agarose
- Aromatic ligand
- Stable in all commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride
- Negligible volume variation due to changes in pH or ionic strength
Octyl Sepharose® CL-4B and Phenyl Sepharose® CL-4B are covalently coupled to a cross-linked 4% agarose matrix by ether linkage, resulting in hydrophobic gels with minimal leakage and no ionic properties.
Octyl Sepharose® 4 Fast Flow is another aliphatic media that provides different and complimentary selectivity. It too is made of agarose beads derivatized via uncharged, chemically stable ether linkages.
Octyl Sepharose® CL-4B and Phenyl Sepharose® CL-4B are covalently coupled to a cross-linked 4% agarose matrix by ether linkage, resulting in hydrophobic gels with minimal leakage and no ionic properties.
Phenyl Sepharose® 6 Fast Flow is available with low or high levels of phenyl substitution differing in selectivity, efficiency, and binding capacity. Both low and high sub media are made of highly cross-linked agarose beads derivatized with phenyl groups via uncharged, chemically stable ether linkages.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...