Lysosomal enzymes containing one or two mannose 6-phosphate (man6P) moieties are moved about in the cell by two distinct but interconnected cycles by means of 300 kDa cation-independent mannose 6-phospate receptors (MPR). MPR cycles and transports newly synthesized lysosomal enzymes between the TGN and late endosomes / early lysosomes. It also cycles and transports extracellular lysosomal enzymes between the plasma membrane and early endosomes via clathrin coated vesicles. The bulk of the MPR protein is in the extracellular/lumenal domain. The entire pool of MPRs cycles between these cellular compartments every 3 hours. The steady state distribution of MPR's is predominantly within late endosomes, fewer in the trans Golgi network and ~5-10 % at the cell surface. In addition to its man6P binding activity, the MPR contains a separate binding site for the type II insulin-like growth factor and is capable of binding both man6P and IGF-II simultaneously. An ~240 kDa soluble, truncated form, representing the extracellular domain of the protein, has also been found circulating in serum and is capable of binding both ligands.
For ICC: Use at a dilution of 1:150 - 1:250. For WB: Use at an assay dependent dilution. Predicted molecular weight: 31 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Type: Primary
Antigen: MPR46
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Chicken
Isotype: IgY
Reactivity: Human
