Neutrophil elastase colorimetric drug discovery kit, Enzo Life Sciences
Supplier: Enzo Life Sciences
It is a complete assay system designed to screen inhibitors of neutrophil elastase, providing enough material to perform at least 96 assays. Neutrophil elastase, also known as leukocyte elastase (LE), is a serine protease found in polymorphonuclear neutrophils (PMNs) that functions in inflammation. It can cleave a variety of proteins, including fibrous elastin, collagen, clotting factors, TNF-α, and α1-antichymotrypsin. It has been shown to be taken up by tumor cells and accelerate tumor growth through its degradation of insulin receptor substrate-1 (IRS-1). Because of its potential roles in cancer and inflammatory diseases such as emphysema, cystic fibrosis, and atherosclerosis, it is a target for drug discovery. The kit uses the chromogenic substrate MeOSuc-Ala-Ala-Pro-Val-pNA; cleavage of p-nitroaniline (pNA) from the substrate increases absorbance at 405nm. A neutrophil elastase inhibitor, elastatinal, is included for use as a control.
Ordering information: Quantity: 96 assays Contents: Neutrophil elastase (human), (purified) (Prod. No. BML-SE284-9090)(30mU; One U=1μmole/min of MeOSuc-AAPV-pNA at 25°C, pH 8.0. Purity ≥95% (SDS-Page))Storage: -70°C; avoid freeze/thaw cycles.pNA substrate (MeOSuc-AAPV-pNA) (Prod. No. BML-P213-9090)(60μl; 20mM in DMSO)Storage: -70°CpNA calibration standard (p-nitroaniline) (Prod. No. BML-KI106)(1ml; 50μM in assay buffer)Storage: -70°CElastatinal (inhibitor) (Prod. No. BML-PI103-9090)(10μl; 20mM in DMSO)Storage: -70°CNeutrophil elastase assay buffer (Prod. No. BML-KI581)(20ml; 100mM HEPES, pH 7.25, 500mM NaCl, 0.05% Tween-20; liquid in a screw-cap plastic bottle)Storage: -20°C or -70°C96-well microplate (Prod. No. BML-KI101)Storage: Room temperature
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