5-CFDA (5-Carboxyfluorescein diacetate) ≥99% (by HPLC) fluorescent pH indicator

Supplier: Biotium

Synonyms: 5-Carboxy-di-O-acetylfluorescein, 5-CFDA, 5-Carboxyfluorescein diacetate

51018

89139-258EA 151.91 USD

89139-258

5-CFDA (5-Carboxyfluorescein diacetate) ≥99% (by HPLC) fluorescent pH indicator

5-CFDA (5-Carboxyfluorescein diacetate)

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).

Cell permeability: Membrane impermeant
Cellular localization: Fluid phase tracer
pKa 4.8
Colors: Green
λex\λem 492nm\517nm (pH 9, after hydrolysis)
Extinction Coefficient (ε) 78,000 (pH 9, after hydrolysis)
White solid soluble in DMSO
Store at 4°C and protect from light, especially in solution

Formula: C₂₅H₁₆O₉
Storage Temperature: Refrigerator

MDL Number: MFCD00036872
CAS Number: 79955-27-4

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