bisbenzimide H-33342 trihydrochloride (Hoechst 33342) 10 mg/mL in water
Supplier: Biotium
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Danger
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Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258, but either dye can be used to stain live or fixed cell in buffer or medium at 1 ug/mL, with no wash step required.
- For ease of use and safer handling
- λEx/λEm (with DNA) = 350/461 nm
- Light yellow solution
- Store at 4°C and protect from light
Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.
Formula:
C₂₇H₂₈N₆O·3HCl Storage Temperature: Refrigerator |
MDL Number:
MFCD00012679 CAS Number: 23491-52-3 EINECS: 245-691-1 |
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