bisbenzimide H-33342 trihydrochloride (Hoechst 33342) 10 mg/mL in water

Supplier: Biotium

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40046
89139-124EA 318.88 USD
89139-124
bisbenzimide H-33342 trihydrochloride (Hoechst 33342) 10 mg/mL in water
bisbenzimide H-33342 trihydrochloride (Hoechst 33342)

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258, but either dye can be used to stain live or fixed cell in buffer or medium at 1 ug/mL, with no wash step required.


  • For ease of use and safer handling
  • λEx/λEm (with DNA) = 350/461 nm
  • Light yellow solution
  • Store at 4°C and protect from light


Hoechst can be used to stain live bacteria (gram-positive and gram-negative) but in live yeast the staining is weak and not nuclear.

Formula: C₂₇H₂₈N₆O·3HCl
Storage Temperature: Refrigerator
MDL Number: MFCD00012679
CAS Number: 23491-52-3
EINECS: 245-691-1

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