Zinc Chelating Resin for Immobilized Metal Affinity Chromatography Purification, G-Biosciences

Supplier: G-Biosciences

786-287
89131-012EA 191.37 USD
89131-012
Zinc Chelating Resin for Immobilized Metal Affinity Chromatography Purification, G-Biosciences
Protein Purification Protein Purification Resins

G-Biosciences' Zinc Chelating Resin is an immobilized metal affinity chromatography (IMAC) resin that was specifically designed to utilize Zn²⁺ for the purification of zinc binding proteins, including 6x histidine tagged proteins.


G-Biosciences' Zinc Chelating Resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent zinc ion with a capacity of 20-40μmoles Znu²⁺/mL resin. The protein binding capacity is >20 mg protein per mL resin. We have demonstrated binding of >40 mg of a 50 kDa 6X His tagged proteins to a mL of resin.


G-Biosciences' Immobilized Nickel, Cobalt and Copper Chelating Resins are also available. Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by Nickel then Zinc. The capacity of zinc is >20 mg per mL. Specific binding/wash and elution buffers are available.


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