Copper Chelating Resin for Immobilized Metal Affinity Chromatography Purification, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' Copper Chelating Resin is an immobilized metal affinity chromatography (IMAC) resin that was specifically designed to utilize Cu²⁺ for the purification of copper binding proteins, including 6x histidine tagged proteins.
G-Biosciences' Copper Chelating Resin binds to six histidine residues (6x His), a common tag used in protein purification. The resin consists of iminodiacetate coupled to 6% cross-linked agarose beads. The iminodiacetate binds divalent copper ion with a capacity of 20-40 μmoles Cuu²⁺/mL resin. The protein binding capacity is >50 mg protein per ml resin. We have demonstrated binding of >100 mg of a 50 kDa 6X His tagged proteins to a mL of resin.
G-Biosciences' Immobilized Nickel, Cobalt and Zinc Chelating Resins are also available. Cobalt has the highest selectivity followed by Zinc, Nickel then Copper, but has the lowest loading capacity. Copper has the highest loading capacity, followed by Nickel then Zinc. The capacity of copper is >50 mg per mL. Specific binding/wash and elution buffers are available.
Ordering information: Accessories available.
Learn more
About VWR
Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...