Glycoprotein Staining Kit, G-Biosciences
Supplier: G-Biosciences
G-Biosciences offers the Glycoprotein Staining kit for the highly sensitive detection of glycoproteins following gel electrophoresis or protein transfer to nitrocellulose membranes
G-Biosciences' Glycoprotein Staining kit uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied oxidizing agent first oxidizes the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive Glyco-Stain Solution forming Schiff bonds and producing strong magenta color bands.
G-Biosciences' Glycoprotein Staining kit is supplied with an enhanced Coomassie stain, called RAPIDstain™, that enhances the glycoprotein staining. RAPIDstain™ can also be used after glycoprotein staining to detect non-glycosylated proteins. The kit is sufficient for 10 mini gels (8 x 8 cm) or 20 nitrocellulose membrane (8 x 8 cm).
The Glycoprotein Staining kit is highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included. In addition, the kit allows for the detection of glycosylated and non-glycosylated proteins on a single gel or membrane.
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