FOCUS™ SubCell, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' FOCUS™ SubCell is for the total subcellular fractionation of cells and mammalian tissue into enriched fractions of nuclear, mitochondrial, cytosolic and membrane proteins.
The resulting mitochondrial fraction can be subsequently separated into heavy and light fractions by gradient centrifugation and the resulting mitochondria are >90% active and have intact inner and outer membranes.
The kit includes reagents for optional steps that minimize contaminations of the nuclear fraction by cytoplasmic elements.
Suitable for cultured animal cells and adaptable for animal tissues. Designed for 50 preps, where one prep is equivalent to 2x10^7 mammalian cells or 100 mg mammalian tissue.
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