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HisTag® Antibody HRP Conjugate Kit, MilliporeSigma

Supplier: MilliporeSigma

71840-3

80017-612EA 708.78 USD

80017-612

HisTag® Antibody HRP Conjugate Kit, MilliporeSigma

Assays Protein Assays

The HisTag® Antibody HRP Conjugate kit contains a horseradish peroxidase-conjugated HisTag® Monoclonal Antibody that is useful as a single detection reagent, thus eliminating cross-reactivity associated with secondary antibody reagents

Using standard HRP detection methods, as little as 1 ng protein can be detected on dot blots. Cross-reactivity to bacterial, insect, yeast or mammalian cell proteins is negligible.

It recognises five consecutive histidine residues regardless of the surrounding amino acid context. The purified antibody conjugate produces a strong signal at a 1:1000 to 1:2000 dilution on Western blots and dot blots. The HisTag® Antibody HRP Conjugate also detects the recombinant marker bands in the Novagen Perfect Protein™ Western Markers for convenient visualisation of accurate internal standards on Western blots.

Ordering information: The package size provides enough conjugate for 25-50 blots and includes 5% Alkali-soluble Casein for the blocking reagent.

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