Sheep Peste Des Petits Ruminants Virus Antibody IgG Titer Serologic Assay Kit
Supplier: ReVacc Scientific
This kit is developed for serologic test for sheep IgG titer of Anti-Peste Des Petits Ruminants Virus (PPRV) antibody in serum/plasma or purified human antibody (monoclonal and polyclonal) in vitro.
- This kit was tested to bind to convalescent sheep sera (n=9) with sensitivity of 100%, specificity of 100%
This kit is developed for a standard indirect-ELISA format, providing a rapid detection of anti-PPRV sheep IgG in serum. The kit consists of high-bind pre-coated, detachable 96-well plate, Positive control, Negative Control, an HRP-Anti-sheep IgG secondary antibody, TMB and dilution/wash/stop buffer.
Use protocol (four simple steps):
1. Detach wells from plate based on your samples and keep at room temperature. Dilute your samples, positive and negative controls in sample dilution buffer and add them to wells (0.1 ml/well). Incubate at 37 °C for 1 h or room temperature for 1.5 h. Wash wells four times by wash solution (0.3 ml).
2. Dilute the Secondary HRP antibody by wash solution and add to each well (0.1 ml). Incubate at room temperature for 0.5 to 1 h. Avoid light by foil. Wash wells as mentioned.
3. Add TMB to each well (0.1 ml) and incubate at room temperature for 5 to 30 minutes.
4. Stop the substrate reaction by adding stop solution (0.1 ml/well). Absorbance (OD) is calculated as the absorbance at 450 nm. The OD Value reflects the amount of antibody bound.
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