NEXTFLEX™ 16S V3-V4 Amplicon-Seq Kits

Supplier: REVVITY HEALTH SCIENCES, INC.

NOVA-4204-04
77448-114EA 3829.54 USD
77448-114
NEXTFLEX™ 16S V3-V4 Amplicon-Seq Kits
Nucleic Acid Reagents Next Generation Sequencing Reagents

The NEXTFLEX™ 16S V3-V4 Amplicon-Seq Kit is designed to prepare multiplexed amplicon libraries that span the hypervariable domains three to four (V3-V4) of microbial 16S ribosomal RNA (rRNA) genes.


  • Fast and easy library prep protocol
  • Low input - only 1ng of input required
  • Multiplex up to 384 libraries
  • Custom sequencing primers are not required
  • Automation protocols are now available for the Sciclone® NGS and NGSx Workstations to automate your 16S sequencing
  • Functionally tested with Illumina® sequencing platforms


Optimized protocol offers lower PCR bias and fewer off-target reads
The NEXTFLEX 16S V3-V4 Amplicon-Seq Kit incorporates a second PCR step in the protocol for the addition of the sample-specific index which reduces off-target reads encountered during amplicon sequencing.


384 Barcodes available for cost-effective 16S V3-V4 rRNA sequencing
The NEXTFLEX 16S V3-V4 Amplicon-Seq Library Prep Kit can be used for multiplexing up to 384 samples, to greatly reduce the cost of sequencing. The barcodes are offered in sets of 48 or 96 barcodes and supplied with 2 reactions worth of each barcode.


Automate your 16S V4 library prep to increase throughput and reduce errors
A NEXTFLEX® automation protocol is now available on the Sciclone® NGS and NGSx workstations to help labs increase their throughput and reduce human errors.


Certifications: Manufactured in ISO 9001 Facility


Caution: For Research Use Only. Not for use in diagnostic procedures.

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