EnzyFluo™ Farnesyltransferase Activity Assay Kit

Supplier: BIOASSAY SYSTEMS

EFTS-400
77441-680EA 383.9 USD
77441-680
EnzyFluo™ Farnesyltransferase Activity Assay Kit
Assays Enzyme Assays

For quantitative determination of FTase enzyme activity in biological samples.


  • Safe and convenient
  • Non-radioactive assay
  • 'Mix-incubate-measure' type assay
  • No wash and reagent transfer steps are involved
  • Sensitive and accurate
  • Linear detection range 0.024 to 3.2 U/L FTase in a 384-well plate assay
  • High-throughput
  • Can be readily automated to assay thousands of samples per day


FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).

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