HisCube HisCube Ni-Indigo MagBead Protein Purification MINI Kit

Supplier: CUBE BIOTECH

80201
77393-516EA 938.33 USD
77393-516
HisCube HisCube Ni-Indigo MagBead Protein Purification MINI Kit
Protein Purification Protein Purification Magnetic Beads

The HisCube MagBead protein purification MINI kit is designed to meet the demands of your laboratory for efficient and high-quality small-scale His-tag protein purifications.


  • Usage: Enrichment of phosphopeptides, specific binding and purification of 6× His-tagged proteins
  • Specificity: Affinity to phosphorylated biomolecules
  • Bead ligand: Zr-NTA
  • Bead size: 30 µm
  • Filling quantity: Delivered as a 25% suspension
  • Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
  • pH stability: 2 to 14
  • Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile


Our team of experts has put their extensive knowledge and experience in His-tag purifications into developing this kit, resulting in a streamlined protocol that is easy to follow. The included MagBead separator is a testament to our commitment to delivering a comprehensive and user-friendly solution. The HisCube INDIGO-Ni MagBead MINI kit contains our in-house developed PureCube INDIGO Ni-MagBeads.


The significant benefit of INDIGO is its tolerance for chelating and reducing agents. While these can significantly assist His-tag purifications, conventional His-tag affinity beads do not tolerate them. EDTA is a chelator often used in eukaryotic cell buffers because it has protease-inhibiting functions. DTT can dissolve protein aggregates that could potentially hinder the access of the purification beads to the His-tagged proteins. However, both chemicals are now usable because of INDIGO. As a result, they are a part of the kit.

Order Now

Learn more

About VWR

Avantor is a vertically integrated, global supplier of discovery-to-delivery solutions for...

Learn more About VWR