Nanodisc MSP1D1-dH5-His DMPG
Supplier: CUBE BIOTECH
This product is a pre-assembled nanodisc. Its intended purpose is to stabilize a cell-free expressed membrane protein.
- Usage: Enrichment of phosphopeptides, specific binding and purification of 6× His-tagged proteins
- Specificity: Affinity to phosphorylated biomolecules
- Bead ligand: Al-NTA
- Bead size: 40 µm
- Filling quantity: Delivered as a 50% suspension
- Matrix: 7.5% crosslinked agarose
- Chelator stability: Stable in buffer containing 10 mM DTT and 1 mM EDTA
- pH stability: 2 to 14
- Other stabilities: 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences for the stabilization of membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 dH5 (His-tagged) creates nanodiscs of ∼7 to 8 nm in diameter.
This product contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.
Delivery information: Shipping temperature: Dry-Ice; storage of reconstituted protein: −80 °C, avoid freeze-thaw cycles.
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