Nanodisc Assembly Kit MSP1D1 POPC
Supplier: CUBE BIOTECH
This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.
- Purity: >90% (determined by SDS-PAGE)
- Number of amino acids: 277
- Molecular mass: 32600 Da
- Extinction coefficient (in water) ε280: 29400 M⁻¹ cm⁻¹
- Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
- Sequence (Human origin): GHHHHHHH DYDIPTT ENLYFQG STFSKLREQLG PVTQEFWDNLEKETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFKVSFLSALEEYTKKLNTQ
- Affinity tag: N-Terminal His-tag
Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences to stabilize membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 creates nanodiscs of ∼9 to 10 nm in diameter.
With this assembly kit both cellular as well as cell-free expressed proteins can be stabilized in MSP nanodiscs.
Delivery information: Shipping temperature: Room temperature; storage of lyophilized copolymer: −20 °C for several years; storage of reconstituted protein: 2 - 8 °C for several day.
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