Nanodisc Assembly Kit MSP1D1-His POPC

Supplier: CUBE BIOTECH

26213
77392-688PK 407.88 USD
77392-688
Nanodisc Assembly Kit MSP1D1-His POPC
Protein Expression Protein Expression Reagents

This product is a MSP nanodisc assembly kit. It contains a vial of lyophilized membrane scaffold protein MSP1D1 (His-tagged) and a vial of DMPC or POPC phopshospholipid to assemble a MSP nanodisc.


  • Origin of MS protein: Human
  • Purity: >90% (determined by SDS-PAGE)
  • Number of amino acids: 201 (human origin), 198 (Mouse origin)
  • Molecular mass: 23329 Da (human origin), 22948 Da (mouse origin)
  • Extinction coefficient (in water) ε280: 25440 M⁻¹ cm⁻¹ (Human origin), 20970 M⁻¹ cm⁻¹ (mouse origin)
  • Buffer: 20 mM TRIS pH 7.4; 100 mM NaCl; 0.5 mM EDTA
  • Sequence (Human origin): GLKLLDNWDSVTSTFSKLREQLG PVTQEFWDNLE KETEGLRQEMS KDLEEVKAKVQ PYLDDFQKKWQEEMELYRQKVE PLRAELQEGARQKLHELQEKLS PLGEEMRDRARAHVDALRTHLA PYSDELRQRLAARLEALKENGG ARLAEYHAKATEHLSTLSEKAK PALEDLRQGLL PVLESFK VSFLSALEEY TKKLNTQ
  • Affinity tag: None


Membrane scaffold proteins derive from apolipoprotein (apo; A-I). They are used in membrane protein sciences to stabilize membrane proteins in a lipid environment after the removal of the cell membrane. The size of the nanodisc is determined by the MSP that was used to create the disc. MSP1D1 (His-tagged) creates nanodiscs of ∼9 to 10 nm in diameter.


With this assembly kit both cellular as well as cell-free expressed proteins can be stabilized in MSP nanodiscs. Just use the corresponding protocol for each purpose (see below). The MSP protein contains a His-tag, keep that in mind when choosing the affinity tag for your membrane protein.


Delivery information: Shipping temperature: Room temperature; storage of lyophilized copolymer: −20 °C for several years; storage of reconstituted protein: 2 to 8 °C for several day.

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