PureCube DIBMA 12, Screening Kit, HEPES
Supplier: CUBE BIOTECH
DIBMA stands for diisobutylene maleic acid copolymer, and is an extensively researched polymer. The fundamental properties of DIBMA include the absence of light absorption at a wavelength >250 nm, a crucial factor for protein detection via absorbance, and a high resistance to divalent cations.
- Usage: Protein solubilization
- Formula weight: 12000 g/mol
- pH: 7.5 in buffer
- dn/dc: 1.35 M⁻¹
- Solubility >10% in H₂O
- Absorbance at 280 nm: <0.3 (1% solution)
- Mg²⁺ Tolerance: Depending on DIBMA product, Increased with less charged DIBMAs
- Ca²⁺ Tolerance: Depending on DIBMA product, Increased with less charged DIBMAs
With diisobutylene-maleic acid (DIBMA), you can directly extract membrane proteins from cells without an intermediate step of detergent solubilization, like with SDS, which would usually interfere with the protein's function. Another advantage of DIBMA as a tool for protein solubilization is the lack of an absorbance maxima at 280 nm. In comparison to SMAs, this would usually interfere with protein quantification, as aromatic amino acids absorb at the same spectrum. PureCube DIBMA is lyophilized from two different buffer solutions (HEPES or TRIS) to ensure a stable pH of 7.5, which is ideal for most protein solubilizations.
Delivery information: Shipping temperature: Ambient temperature; Storage of lyophilized copolymer: –20 °C for several years; Storage of dissolved copolymer: 2 to 8 °C for several day.
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