Human IFN Gamma Receptor Beta/AF-1 ELISA Kit

Supplier: ANTIBODIES.COM LLC

A311291-96
77201-008EA 555.5 USD
77201-008
Human IFN Gamma Receptor Beta/AF-1 ELISA Kit
Assays ELISAs

Human IFN gamma Receptor beta/AF-1 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human IFN gamma Receptor beta/AF-1 in serum, plasma, and other biological fluids.


  • Higher throughput: Get results in just 90 minutess, with a single wash step
  • Detection Range: 0.8 to 12.8 ng/ml
  • Sensitivity: 0.02 ng/ml
  • Sample Type: Serum, plasma or other biological fluids
  • Assay precision: Intra assay: CV <8%, Inter assay: CV <10%


Human IFN gamma Receptor beta/AF-1 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human IFN gamma Receptor beta/AF-1 in serum, plasma or other biological fluids. An antibody specific for IFN gamma Receptor beta/AF-1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IFN gamma Receptor beta/AF-1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-IFN gamma Receptor beta/AF-1 antibody, which also binds the IFN gamma Receptor beta/AF-1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-IFN gamma Receptor beta/AF-1 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-IFN gamma Receptor beta/AF-1 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of IFN gamma Receptor beta/AF-1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of IFN gamma Receptor beta/AF-1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.


Ordering information: This is a ready to use ELISA kit that contains: Pre-coated 96 well microplate, standard solution, standard diluent, biotinylated detection antibody, steptavidin-HRP, wash buffer (25X), substrate solution A, substrate solution B, stop solution, plate sealers, foil pouch, and protocol manual.

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