Mouse Caveolin-1 ELISA Kit
Supplier: ANTIBODIES.COM LLC
Mouse Caveolin-1 ELISA kit is a 90 minute sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse Caveolin-1 in serum, plasma, and other biological fluids.
- Higher throughput: Get results in just 90 minutes, with a single wash step
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse Caveolin-1 ELISA kit (A313603) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse Caveolin-1 in serum, plasma or other biological fluids. An antibody specific for Caveolin-1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Caveolin-1 present in each sample is bound to the wells by the immobilized antibody. Biotinylated anti-Caveolin-1 antibody, which also binds the Caveolin-1 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Caveolin-1 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Caveolin-1 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Color develops in proportion to the amount of Caveolin-1 captured in each well. The color development is stopped by addition of stop solution which changes the color from blue to yellow and the intensity of the color is then measured. The concentration of Caveolin-1 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.
Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, positive control, negative control, HRP-conjugated detection antibody, sample diluent, substrate solution A, substrate solution B, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.
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