PH.D.-7 Phage Display Peptide Library Kit v2, New England Biolabs
Supplier: New England Biolabs (NEB)
The Ph.D.−7 phage display peptide library kit v2 is based on a combinatorial library of random heptapeptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The peptide is followed by a short spacer (Gly-Gly-Gly) which directly precedes the wild-type pIII sequence. The library consists of ~1E09 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
- Ready to use complex phage library (~1E09 clones)
- Monoclonal antibody and protein G magnetic beads included for new control panning experiment
- Panning against mouse mAb yields more reliable epitope results than former target protein
- Does not require helper phage for amplification
- Inherent link between phenotype and genotype allows screening of billions of clones in a single tube
The Ph.D.−7 phage display peptide library kit v2 contains the Ph.D.−7 phage display peptide library, a DYKDDDDK mouse monoclonal antibody and protein G magnetic beads for a panning control experiment, and enough −96 gIII sequencing primer for >50 sequencing reactions.
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