Control LAMP Primer Mix (rActin), New England Biolabs
Supplier: New England Biolabs (NEB)
The Control LAMP Primer Mix (rActin) targets actin RNA at an exon-exon junction. It is a LAMP primer set (F3, B3, FIP, BIP, LF, LB) that amplifies rActin to ensure the absence of inhibition from human nucleic acid templates in a LAMP reaction.
- Provided at 10X concentration
- Optimized mix of 6 primers (F3, B3, FIP, BIP, LF, LB) targets actin RNA at an exon-exon junction
- Can be used in a LAMP control reaction to verify assay and reagent performance
- Should return a positive amplification readout in a LAMP protocol
Control LAMP primer sequences: rActin (5'→ 3')
rActin primer set sequence:
ACTB-F3 AGTACCCCATCGAGCACG
ACTB-B3 AGCCTGGATAGCAACGTACA
ACTB-FIP GAGCCACACGCAGCTCATTGTATCACCAACTGGGACGACA
ACTB-BIP CTGAACCCCAAGGCCAACCGGCTGGGGTGTTGAAGGTC
ACTB-LF TGTGGTGCCAGATTTTCTCCA
ACTB-LB CGAGAAGATGACCCAGATCATGT
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