Sce PUS1

Supplier: New England Biolabs (NEB)

M0526S
76459-498EA 88 USD
76459-498
Sce PUS1
Enzymes

Sce Pseudouridine Synthase I (Sce PUS1) is expressed as a 6X His tagged recombinant protein and converts Uridine to Pseudouridine in RNA.


  • Sce PUS1 isomerizes uridine to pseudouridine in predominantly single-stranded RNA in vitro
  • Sce PUS1 is a stand-alone enzyme and does not require an RNA guide or co-factor to modify RNA


Sce PUS1 has a preference for Uridines in single stranded RNA regions over Uridines in double-stranded RNA. Optimal substrate is an unstructured RNA that is 15 nt long or longer. Sequence-specific pseudouridine modification by Sce PUS1 is an alternative to randomly incorporated modified nucleosides by RNA polymerases.

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