EnGen Sau Cas9
Supplier: New England Biolabs (NEB)
EnGen Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA.
- Longer 5' NNGRRT 3' PAM sequence allows for higher specificity
- Two nuclear localization signals for improved transport into the nucleus
- High concentration liquid format option
- Compatible with the EnGen mutation detection kit
Targeting requires a ~100 nucleotide guide RNA (gRNA) complementary to the target site, as well as a 5' NNGRRT 3' (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. DNA cleavage by EnGen Sau Cas9 occurs ~3 bases 5' of the PAM. EnGen Sau Cas9 has Simian virus 40 (SV40) T antigen nuclear localization signals (NLS) at both the N- and C-termini of the protein. Schematic representation of Sau Cas9 nuclease sequence recognition and DNA cleavage.
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