yDcpS
Supplier: New England Biolabs (NEB)
M0463S
76339-732EA
308
USD
76339-732
yDcpS
Enzymes
yDcpS is the scavenging decapping enzyme from S. cerevisiae that hydrolyzes the phosphodiester bond between the gamma and beta phosphates of m7G-capped mRNA, producing diphosphorylated 5 ends and m7GMP. yDcpS is capable of decapping mRNAs of various lengths, decaps Cap0 and Cap1 with equal efficiency, and the diphosphorylated 5 end it produces is suitable for recapping with Vaccinia capping enzyme.
- Provided at high enzyme concentration to facilitate complete decapping
- Active on capped RNA of various sizes (tested on in vitro capped transcripts from 25 nt to 1400 nt)
- Decapped RNA 5' ends can be recapped with Vaccinia capping system supports tagging of mRNA with desthiobiotin-labeled GTP
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