Q5® Hot Start High-Fidelity 2X Master Mix

Supplier: New England Biolabs (NEB)

M0494L M0494S M0494X
102500-140EA 1013 USD
102500-140 102500-142 76307-458
Q5® Hot Start High-Fidelity 2X Master Mix
Nucleic Acid Reagents End-point PCR Enzymes and Kits

The Q5 Hot start high-fidelity 2X master mix features a high-fidelity, thermostable, hot start DNA polymerase with 3' to 5' exonuclease activity, fused to a processivity-enhancing Sso7d domain to support robust DNA amplification.



  • Highest fidelity amplification (~280X higher than Taq)

  • Ultra-low error rates
  • Superior performance for a broad range of amplicons (from high AT to high GC)
  • Hot start and master mix formats available


The addition of an aptamer-based inhibitor allows room temperature reaction setup. With an error rate ~280-fold lower than that of Taq DNA Polymerase, Q5 Hot start high-fidelity 2X master mix is ideal for cloning and can be used for long or difficult amplicons. The convenient master mix formulation is supplied at a 2X concentration. The mix contains dNTPs, Mg++ and a proprietary broad-use buffer requiring only the addition of primers and DNA template for robust amplification regardless of GC content. When used at the recommended 1X final concentration, the Q5 Hot start high-fidelity master mix contains 2 mM Mg++. Q5 Hot start high-fidelity 2X master mix is unlike typical, lower fidelity PCR enzymes.


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