The protein named TrkB (also named Neurotrophic tyrosine kinase receptor type 2 (NTRK2), GP145-TrkB or Tropomyosin-related kinase B is a receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems and is important in the regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. TrkB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. TrkB is the primary receptor for BDNF (brain-derived neurotrophic factor. TrkB also binds NT4 and NT3 but less efficiently (Reference: www.uniprot.org). Antibody reacts with human and mouse, other species not yet tested but it is predicted to react with rat and chicken TrkB-pS478 based on amino acid homology at the site. Antibody has been shown to be specific for TrkB phosphorylated on serine 478 by phospho-peptide absorption dot blots, and on cell lysates from cell lines induced with retinoic acid and BDNF. Antibody detects a clear band in retinoic acid (RA) and BDNF-treated NSC34 cell lysates at 140 kDa only, indicating that the phosphorylated TrkB receptor is being detected. Additional non-specific bands at lower molecular weight are observed in both lysates with the antibody and these bands have not been characterized.
- Recommeded 0.5 to 2 µg/ml for western blotting and 1 to 5 µg/ml for immunohistochemistry
- TBS is preferred for buffer preparation
Cell lysates or membrane preparations prepared from isolated brain or spinal cord tissues are recommended. Highly purified BSA is recommended for membrane blocking, although this antibody has been shown to work on membranes blocked with skim milk. Antibody has been shown to work on PFA fixed, frozen sections. Other applications have not been tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Type: Primary
Antigen: TrkB
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope: Corresponds to:
472SNDDDSASPLHHIS485 in human TrkB. Phosphorylated Serine target is amino acid number 479 in human.
and
471SNDDDSASPLHHIS484 in Uniprot# Q63604 (NTRK2_RAT) for rat. Phosphorylated Serine target is amino acid 478 in Rat.
Host: Rabbit
Isotype:
Reactivity: Human, Mouse