Human GDNF ELISA Kit, Rockland Immunochemicals

Supplier: Rockland Immunochemical

KOA0180
76237-564EA 760.6 USD
76237-564
Human GDNF ELISA Kit, Rockland Immunochemicals
Assays ELISAs

Human GDNF AccuSignal ELISA Kit


  • Natural and recombinant human GDNF.
  • There is no detectable cross-reactivity with other relevant proteins.
  • Expression system for standard: NSO; Immunogen sequence: R109-I211


Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures.


Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human GDNF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human GDNF standard solution and each sample be measured in duplicate.

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