Human AFP ELISA Kit, Rockland Immunochemicals
Supplier: Rockland Immunochemical
Human AFP AccuSignal ELISA Kit
- Natural and recombinant human AFP.
- There is no detectable cross-reactivity with other relevant proteins.
- Expression system for standard: NSO; Immunogen sequence: R19-V609
Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml, 78pg/ml human AFP standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each human AFP standard solution and each sample is measured in duplicate.
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